Despite his family’s baffling generations-old ban on music, Miguel dreams of becoming an accomplished musician like his idol, Ernesto de la Cruz. Desperate to prove his talent, Miguel finds himself in the stunning and colorful Land of the Dead following a mysterious chain of events. Along the way, he meets charming trickster Hector, and together, they set off on an extraordinary journey to unlock the real story behind Miguel's family history.
| Miguel Rivera (voice) | Anthony Gonzalez |
| Héctor (voice) | Gael García Bernal |
| Ernesto de la Cruz (voice) | Benjamin Bratt |
| Mamá Imelda (voice) | Alanna Ubach |
| Abuelita (voice) | Renée Victor |
| Papá (voice) | Jaime Camil |
| Papá Julio (voice) | Alfonso Arau |
| Tío Oscar / Tío Felipe (voice) | Herbert Siguenza |
| Clerk (voice) | Gabriel Iglesias |
| Plaza Mariachi / Gustavo (voice) | Lombardo Boyar |
| Mamá Coco (voice) | Ana Ofelia Murguía |
| Frida Kahlo (voice) | Natalia Cordova-Buckley |
| Mamá (voice) | Sofía Espinosa |
| Tía Rosita (voice) | Selene Luna |
| Chicharrón (voice) | Edward James Olmos |
| Departures Agent / Additional Voices (voice) | Carla Medina |
| Tía Victoria / Additional Voices (voice) | Dyana Ortelli |
| Tío Berto / Don Hidalgo (voice) | Luis Valdez |
| Emcee (voice) | Blanca Araceli |
| Security Guard (voice) | Salvador Reyes |
| Corrections Officer (voice) | Cheech Marin |
| Arrivals Agent (voice) | Octavio Solis |
| Juan Ortodoncia (voice) | John Ratzenberger |
| Additional Voices (voice) | Lalo Alcaraz |
| Additional Voices (voice) | Carolina Ángel |
| Additional Voices (voice) | Memo Aponte |
| Additional Voices (voice) | Marcela Davison Aviles |
| Additional Voices (voice) | Liliana Barba Meinecke |
| Additional Voices (voice) | Emmanuel Bernal |
| Additional Voices (voice) | David Berón |
| Additional Voices (voice) | Denise Blasor |
| Additional Voices (voice) | Wilma Bonet |
| Additional Voices (voice) | Óscar Bonfiglio |
| Additional Voices (voice) | Alex Castillo |
| Additional Voices (voice) | Gary Carlos Cervantes |
| Additional Voices (voice) | Bernardo Cubria |
| Additional Voices (voice) | Vicki Davis |
| Additional Voices (voice) | Daniel Diaz |
| Additional Voices (voice) | Roberto Donati |
| Additional Voices (voice) | Efrain Figueroa |
| Additional Voices (voice) | Deb Fink |
| Additional Voices (voice) | Emilio Fuentes |
| Additional Voices (voice) | Daniella Garcia |
| Additional Voices (voice) | Libertad García Fonzi |
| Additional Voices (voice) | Mike Gomez |
| Additional Voices (voice) | Lillian Garrett-Groag |
| Additional Voices (voice) | Joshua Guerrero |
| Additional Voices (voice) | Montse Hernandez |
| Additional Voices (voice) | Marabina Jaimes |
| Additional Voices (voice) | Jossara Jinaro |
| Additional Voices (voice) | Christian Lanz |
| Additional Voices (voice) | Constanza Lechuga |
| Additional Voices (voice) | Luisa Leschin |
| Additional Voices (voice) | Ruth Livier |
| Additional Voices (voice) | Maria Dominique Lopez |
| Additional Voices (voice) | Valeria Maldonado |
| Additional Voices (voice) | Richard Miro |
| Additional Voices (voice) | Adrian Molina |
| Additional Voices (voice) | Daniel Edward Mora |
| Additional Voices (voice) | Carlos Moreno Jr. |
| Additional Voices (voice) | Vivianne Nacif |
| Additional Voices (voice) | Adriana Sevan |
| Additional Voices (voice) | Jonathan Nichols-Navarro |
| Additional Voices (voice) | Levi Nunez |
| Additional Voices (voice) | Arthur Ortiz |
| Additional Voices (voice) | Jessica Pacheco |
| Additional Voices (voice) | Juan Pacheco |
| Additional Voices (voice) | Jacqueline Pinol |
| Additional Voices (voice) | James Ponce |
| Additional Voices (voice) | Al Rodrigo |
| Additional Voices (voice) | J. Francisco Rodriguez |
| Additional Voices (voice) | Polo Rojas |
| Additional Voices (voice) | Eduardo Ronan |
| Additional Voices (voice) | Johnny A. Sanchez |
| Additional Voices (voice) | Eddie Santiago |
| Additional Voices (voice) | Melissa Santos |
| Additional Voices (voice) | Luis Solís |
| Additional Voices (voice) | Rosalba Sotelo |
| Additional Voices (voice) | Chris Triana |
| Additional Voices (voice) | Trujo |
| Additional Voices (voice) | Lee Unkrich |
| Additional Voices (voice) | Ruth Zalduondo |
| Additional Voices (voice) | James Zavaleta |
